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 RNA Synthesis Services      

 

     

As an important research tool, RNA oligo is widely used in research applications such as gene function analysis and development of novel therapeutic strategies. GENERAY Biotechnology provides high-quality customized services for RNA synthesis, which can meet the different needs of our customers. RNA synthesis services include: General RNA synthesis, RNA modification and labeling, Chimeric RNA, 2'-OMe-RNA and other antisense RNAs. In order to ensure the high quality of RNA oligo, synthetic RNA strictly implements QC testing standards. Through MS, HPLC and other detection methods to ensure that the final product delivered to customers is high quality RNA oligo.

 

1.siRNA and Single RNA

 

siRNA synthesis is the easiest way to achieve gene silencing in the body or cells, with low cost and high transfection efficiency. It is also the best method for clinical treatment of RNAi. It has irreplaceable advantages in drug development. GENERAY Biotechnology can provide customers with common chemically synthesized siRNAs, and can provide a variety of modified siRNAs according to customer needs. Ordinary chemically synthesized siRNA products are purified by HPLC to completely remove unpaired single strands. Customers only need to dilute with the buffer in the box to perform subsequent 

 

 

1.1 Regular siRNA synthesis pricelist (15-25bp) double-stranded

 

 

Project description

Specifications Purification Way

Delivery Time

Conventional siRNA

 

2 OD

HPLC

5-6 working days

5 OD

HPLC

10 OD

HPLC

Note: Conventional siRNA provides a annealed double strand by default, with two overhangs dT at the 3' end

1.2 Modified siRNA synthesis pricelist (15-25bp) double-stranded

 

 

Project description

Specifications

Purification Way

Delivery Time

5‘FAM/CY3/CY5

 

2 OD

HPLC

5-6 working days

5 OD

HPLC

10 OD

HPLC

5'Biotin/Amino/Phosphate

2 OD

HPLC

5-6 working days

5 OD

HPLC

10 OD

HPLC

5' Thiol/Cholesterol

2 OD

HPLC

5-6 working days

5 OD

HPLC

10 OD

HPLC

agomir/ antagomir

 

2 OD

HPLC

5-6 working days

5 OD

HPLC

10 OD

HPLC

5’FAM 3'BHQ1

2 OD

HPLC

5-6 working days

5 OD

HPLC

10 OD

HPLC

 

 

2. siRNA Package

 

2.1  Regular siRNA Package

 

 

Project description

specifications

siRNA oligo for the target gene

3 sites

2 OD

HPLC

Negative control siRNA oligo

1 pair

1 OD

HPLC

FAM labeled negative control siRNA oligo

1 pair

1 OD

HPLC

RNAi positive control siRNA oligo

1 pair

1 OD

HPLC

 

 

2.2 Modified siRNA Package

 

Project description

specifications

siRNA oligo for the target gene

3 sites

2 OD

HPLC

Negative control siRNA oligo

1 pair

1 OD

HPLC

FAM labeled negative control siRNA oligo

1 pair

1 OD

HPLC

RNAi positive control siRNA oligo

1 pair

1 OD

HPLC

Note: Full chain methoxy modification, terminal cholesterol modification

 

Package description: To ensure that the transfection efficiency is above 80%, the silence efficiency can reach 70%. Otherwise, design and synthesize again for free.

                                                                                                                                            

3、miRNA

 

GENERAY miRNA products mainly include miRNA Mimic(double-stranded annealing products), miRNA Mimic negative control, miRNA inhibitor (RNA single chain) and miRNA inhibitor negative control products。The miRNA products provided by our company are produced under the quality standard of ISO9001:2015 and have passed repeated optimization and rigorous testing, the product quality is highly stable.

 

Project description

Purification Way

Cycle

miRNA Mimics

(Double chain annealing product)

HPLC

5-7 working days

                 miRNA Mimics negative control

HPLC

5-7 working days

miRNA inhibitor (RNA strands)

HPLC

5-7 working days

miRNA inhibitor negative control

HPLC

5-7 working days

Note: (1) All products were purified by HPLC and passed strict quality control

(2) All RNA is produced in the clean workshop to strictly control endotoxin and meet the requirements of cell level and animal level

(3) Provide mass production

(4) Partial modified and long-stranded RNAs are not listed and shall be inquire

 



















 

 

4、shRNA

 

The shRNA expression vector is labeled with antibiotics and can continuously suppress the expression of target genes in cells for weeks or longer. For a known effective siRNA sequence (for example, obtained by screening by siRNA synthesis method), when it is necessary to maintain gene silencing for a long time, the shRNA vector system is recommended, GENERAY Biological is committed to providing the most advanced and convenient shRNA-related tools, and recently launched a new generation of shRNA expression plasmids, an efficient and ready-to-use vector that can continuously generate shRNAs in cells, thereby achieving a long-lasting inhibition of target gene expression. purpose.

 

Project description

Specifications

Cycle

ShRNA Plasmid expression vector (pGPU6)

50μg

2   weeks 

ShRNA Plasmid expression vector (pGPH1)

50μg

2   weeks

ShRNA Plasmid expression vector (pGPU6/Neo)

50μg

2   weeks

ShRNA Plasmid expression vector (pGPH1/Neo)

50μg

2   weeks

ShRNA Plasmid expression vector (pGPU6/Hygro)

50μg

2   weeks

ShRNA Plasmid expression vector (pGPH1/Hygro)

50μg

2   weeks

ShRNA Plasmid expression vector (pGPU6/GFP/Neo)

50μg

2   weeks

ShRNA Plasmid expression vector (pGPH1/GFP/Neo)

50μg

2   weeks

ShRNA Plasmid expression vector (pGPU6/RFP/Neo)

50μg

2   weeks

ShRNA Plasmid expression vector (pGPH1/RFP/Neo)

50μg

2   weeks

ShRNA Plasmid expression vector (Plko.1-puro)

50μg

2   weeks

5、Oligo and other coupling

 

GENERAY Biological can provide PEG, Peptide, chelating agent (NOTA, DOTA, MAG, DTPA) and other small molecule conjugation services with Oligo.

 

Quality Assurance

 

Perfect order service system

 All RNA oligonucleotides were produced under strict SOP & Quality Control

 High resolution Waters chromatography with ESI-MS ensure the purity

 Fully traceable ISO 9001 compliance documentation system

 

 

Result of Delivery

 

Synthetic RNA lyophilized powder

 

COA file

 

Examination Report (MS & HPLC)

 

DNA Production Quality Control Flow Chart

 

RNA Production Quality Control Report-HPLC Primer purity test

 

 

Explanation of test results: In the figure, the area of the peak accounts for 100% . So the test is qualified.
Eligibility criteria: The main peak area is 90% or more.

 

 

RNA Production Quality Control Report-MS

 

 

MS test result description: Since RNA is double-stranded, we provide the theoretical molecular weight and actual molecular weight of the two strands. The test results are shown in the attachment. For example, the first double-stranded RNA test report has theoretical molecular weights of 6725.11 and 6622.08, and actual molecular weights of 6724.7 and 6621.7. So the test is qualified.
Eligibility criteria: The measured molecular weight and the molecular weight less than 0.1% difference theory.


 

Have a Question?

Order Online:rna@generay.com.cn

By Phone: 021-67840801/67840802